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adherent cell line  (ATCC)


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    ATCC adherent cell line
    Adherent Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 4831 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+line+adherence/BxPC-3/us12630843-258-12-7
    Average 99 stars, based on 4831 article reviews
    adherent cell line - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Inhibition:

    Article Title: Interaction of moraxella catarrhalis with epithelial cells, extracellular matrix proteins and the complement system
    Article Snippet: .. Cell Line Adherence Inhibition Assay Chang conjunctival cells (ATCC CCL 20.2) were cultured in RPMI 1640 medium (Gibco BRL, Life Technologies, Paisley, Scotland) supplemented with 10% fetal calf serum, 2 mM L-glutamine, and 12 μg of gentamicin/ml. .. On the day before adherence inhibition experiments, cells were harvested, washed twice in gentamicin-free RPMI 1640, and added to 96 well tissue culture plates (Nunc) at a final concentration of 104 cells/well in 200 μl of gentamicin-free culture medium.

    Cell Culture:

    Article Title: Interaction of moraxella catarrhalis with epithelial cells, extracellular matrix proteins and the complement system
    Article Snippet: .. Cell Line Adherence Inhibition Assay Chang conjunctival cells (ATCC CCL 20.2) were cultured in RPMI 1640 medium (Gibco BRL, Life Technologies, Paisley, Scotland) supplemented with 10% fetal calf serum, 2 mM L-glutamine, and 12 μg of gentamicin/ml. .. On the day before adherence inhibition experiments, cells were harvested, washed twice in gentamicin-free RPMI 1640, and added to 96 well tissue culture plates (Nunc) at a final concentration of 104 cells/well in 200 μl of gentamicin-free culture medium.

    Serial Dilution:

    Article Title: High-throughput single-cell analysis reveals Omp38-specific monoclonal antibodies that protect against Acinetobacter baumannii infection
    Article Snippet: .. The degree of bacterial adherence to A549 cells was quantified via the serial dilution method after the removal of external nonadherent bacteria. (B) Intracellular proliferation of A. baumannii ATCC 17978 in the presence of the mAb C3 or the isotype control. ..

    Bacteria:

    Article Title: High-throughput single-cell analysis reveals Omp38-specific monoclonal antibodies that protect against Acinetobacter baumannii infection
    Article Snippet: .. The degree of bacterial adherence to A549 cells was quantified via the serial dilution method after the removal of external nonadherent bacteria. (B) Intracellular proliferation of A. baumannii ATCC 17978 in the presence of the mAb C3 or the isotype control. ..

    Control:

    Article Title: High-throughput single-cell analysis reveals Omp38-specific monoclonal antibodies that protect against Acinetobacter baumannii infection
    Article Snippet: .. The degree of bacterial adherence to A549 cells was quantified via the serial dilution method after the removal of external nonadherent bacteria. (B) Intracellular proliferation of A. baumannii ATCC 17978 in the presence of the mAb C3 or the isotype control. ..

    In Vitro:

    Article Title: Screening of small molecules attenuating biofilm formation of Acinetobacter baumannii by inhibition of ompA promoter activity.
    Article Snippet: natives against drug-resistant pathogens.. Outer membrane protein A (OmpA) plays a versatile role in the pathogenesis and antimicrobial resistance of Acinetobacter baumannii.. Therefore, OmpA is an innovative target for anti-virulence therapy against A. baumannii.

    Produced:

    Article Title: Mechanisms Underlying the Virulence Regulation of Vibrio alginolyticus ND-01 pstS and pstB with a Transcriptomic Analysis
    Article Snippet: .. Recent studies have shown that the loss of a single gene can change the landscape of bacterial transcriptomes [ ]. pstS loss significantly decreased bacterial adherence and invasion into A549 cells and increased A549 cell viability. pstS loss also reduced the motility and biofilm-forming ability of A . baumannii , and the minimum lethal dose required by A. baumannii ATCC 17978 Δ pstS was lower compared to the wild type; thus, the loss of the phosphate sensor PstS produced a decrease in A. baumannii pathogenesis, supporting its role as a virulence factor [ ]. ..



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    (A) Hierarchical clustering dendrograms of FBS conditions based on differential gene expression profiles <t>in</t> <t>MRC-5</t> (left), Jurkat (middle), and THP-1 (right) cells. Clustering was performed using the number of differentially expressed genes (DEGs; |log2FC| > 0.6, adjusted p-value < 0.05) as a distance metric. Horizontal red lines indicate the clustering threshold of 100 DEGs, defining distinct FBS clusters. MRC-5 and THP-1 cells show 4 distinct clusters, while Jurkat cells display 5 clusters, revealing cell type-specific responses to FBS alternatives. (B) Module size distributions from WGCNA for MRC-5 (left), Jurkat (middle), and THP-1 (right) cells. Bar colors represent distinct co-expression modules identified using signed networks with Pearson correlation (soft-thresholding power selected to achieve scale-free topology R ≥ 0.8; minimum module size = 30 genes; merge cut height = 0.25). MRC-5 cells: 7 modules (turquoise: 4,728 genes; blue: 3,580; brown: 2,417; grey: 936; yellow: 634; green: 447; red: 234). Jurkat cells: 7 modules (turquoise: 4,234; blue: 3,635; grey: 2,289; brown: 1,464; yellow: 660; green: 240; red: 204). THP-1 cells: 5 modules (turquoise: 4,201; blue: 4,16; grey: 2,478; brown: 2,184; yellow: 434). (C) Module-trait relationship heatmaps showing Pearson correlations between module eigengenes and FBS cluster assignments for MRC-5 (left), Jurkat (middle), and THP-1 (right) cells. Each cell displays the correlation coefficient (color scale: blue = -0.5, white = 0, red = 0.5) and corresponding p-value. Significant correlations (p < 0.05, calculated using corPvalueStudent) indicate modules with expression patterns specifically associated with FBS clusters. Notable associations include: MRC-5 turquoise module with Cluster 1 (r = - 0.45, p = 3.22×10 ); Jurkat red module with Cluster 2 (r = 0.76, p = 1.38×10 ); THP-1 yellow module with Cluster 1 (r = 0.65, p = 2.54×10 8).
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    Image Search Results


    (A) Hierarchical clustering dendrograms of FBS conditions based on differential gene expression profiles in MRC-5 (left), Jurkat (middle), and THP-1 (right) cells. Clustering was performed using the number of differentially expressed genes (DEGs; |log2FC| > 0.6, adjusted p-value < 0.05) as a distance metric. Horizontal red lines indicate the clustering threshold of 100 DEGs, defining distinct FBS clusters. MRC-5 and THP-1 cells show 4 distinct clusters, while Jurkat cells display 5 clusters, revealing cell type-specific responses to FBS alternatives. (B) Module size distributions from WGCNA for MRC-5 (left), Jurkat (middle), and THP-1 (right) cells. Bar colors represent distinct co-expression modules identified using signed networks with Pearson correlation (soft-thresholding power selected to achieve scale-free topology R ≥ 0.8; minimum module size = 30 genes; merge cut height = 0.25). MRC-5 cells: 7 modules (turquoise: 4,728 genes; blue: 3,580; brown: 2,417; grey: 936; yellow: 634; green: 447; red: 234). Jurkat cells: 7 modules (turquoise: 4,234; blue: 3,635; grey: 2,289; brown: 1,464; yellow: 660; green: 240; red: 204). THP-1 cells: 5 modules (turquoise: 4,201; blue: 4,16; grey: 2,478; brown: 2,184; yellow: 434). (C) Module-trait relationship heatmaps showing Pearson correlations between module eigengenes and FBS cluster assignments for MRC-5 (left), Jurkat (middle), and THP-1 (right) cells. Each cell displays the correlation coefficient (color scale: blue = -0.5, white = 0, red = 0.5) and corresponding p-value. Significant correlations (p < 0.05, calculated using corPvalueStudent) indicate modules with expression patterns specifically associated with FBS clusters. Notable associations include: MRC-5 turquoise module with Cluster 1 (r = - 0.45, p = 3.22×10 ); Jurkat red module with Cluster 2 (r = 0.76, p = 1.38×10 ); THP-1 yellow module with Cluster 1 (r = 0.65, p = 2.54×10 8).

    Journal: bioRxiv

    Article Title: Towards molecular-based functional classification of fetal bovine serum

    doi: 10.64898/2026.03.16.712020

    Figure Lengend Snippet: (A) Hierarchical clustering dendrograms of FBS conditions based on differential gene expression profiles in MRC-5 (left), Jurkat (middle), and THP-1 (right) cells. Clustering was performed using the number of differentially expressed genes (DEGs; |log2FC| > 0.6, adjusted p-value < 0.05) as a distance metric. Horizontal red lines indicate the clustering threshold of 100 DEGs, defining distinct FBS clusters. MRC-5 and THP-1 cells show 4 distinct clusters, while Jurkat cells display 5 clusters, revealing cell type-specific responses to FBS alternatives. (B) Module size distributions from WGCNA for MRC-5 (left), Jurkat (middle), and THP-1 (right) cells. Bar colors represent distinct co-expression modules identified using signed networks with Pearson correlation (soft-thresholding power selected to achieve scale-free topology R ≥ 0.8; minimum module size = 30 genes; merge cut height = 0.25). MRC-5 cells: 7 modules (turquoise: 4,728 genes; blue: 3,580; brown: 2,417; grey: 936; yellow: 634; green: 447; red: 234). Jurkat cells: 7 modules (turquoise: 4,234; blue: 3,635; grey: 2,289; brown: 1,464; yellow: 660; green: 240; red: 204). THP-1 cells: 5 modules (turquoise: 4,201; blue: 4,16; grey: 2,478; brown: 2,184; yellow: 434). (C) Module-trait relationship heatmaps showing Pearson correlations between module eigengenes and FBS cluster assignments for MRC-5 (left), Jurkat (middle), and THP-1 (right) cells. Each cell displays the correlation coefficient (color scale: blue = -0.5, white = 0, red = 0.5) and corresponding p-value. Significant correlations (p < 0.05, calculated using corPvalueStudent) indicate modules with expression patterns specifically associated with FBS clusters. Notable associations include: MRC-5 turquoise module with Cluster 1 (r = - 0.45, p = 3.22×10 ); Jurkat red module with Cluster 2 (r = 0.76, p = 1.38×10 ); THP-1 yellow module with Cluster 1 (r = 0.65, p = 2.54×10 8).

    Article Snippet: The adherent human lung fibroblast cell line MRC-5 (CCL-171), the suspension human monocytic cell line THP-1 (TIB-202), and the human acute T-cell leukemic cell line Jurkat Clone E6-1 (TIB-152) were purchased from American Type Culture Collection (ATCC, USA).

    Techniques: Gene Expression, Expressing